Farmerline Impact Case Study: Grow-ing Empowerment

Women farmers in the Northern Region of Ghana lack access to the financial resources and agricultural inputs needed to adapt to climate change. If women had access to the same agricultural resources as men, agricultural yields could increase by 20-30% and the total number of hungry people would decrease by 12-17%. To address this gender gap, MEDA started its GROW project providing information, training, and resources to female farmers. To reach its goal of 20,000 farmers, MEDA needs a more efficient way to regularly distribute information than bi-annual in-person workshops

Farmerline Impact Case Study: The Ghanian Ministry of Food and Agriculture

A case study to be featured on Farmerline’s website analyzes the impact of Farmerline’s business partnerships on both the businesses and farmers. This case study examines how partners are using Farmerline’s services to and what the impact this has on the livelihoods of farmers

Farmerline Impact Case Study: Tracing The Source

A case study to be featured on Farmerline’s website analyzes the impact of Farmerline’s business partnerships on both the businesses and farmers. This case study examines how partners are using Farmerline’s services to and what the impact this has on the livelihoods of farmers

Farmerline Impact Case Study: Cocoa Linked

A case study to be featured on Farmerline’s website analyzes the impact of Farmerline’s business partnerships on both the businesses and farmers. This case study examines how partners are using Farmerline’s services to and what the impact this has on the livelihoods of farmers

Farmerline Impact Case Study: Connecting a Movement

A case study to be featured on Farmerline’s website analyzes the impact of Farmerline’s business partnerships on both the businesses and farmers. This case study examines how partners are using Farmerline’s services to and what the impact this has on the livelihoods of farmers

Halo - A Personal IoT Air Monitor Powered by Harvested Energy

Urban air pollution leads to widespread respiratory illness and millions of deaths annually. PM2.5, particulate matter with a diameter less than 2.5 micrometers, is the product of many common combustion reactions and poses a particularly serious health risk. Its small size allows it to penetrate deep into the lungs and enter the bloodstream. Existing air quality monitors are aimed at scientific research, di↵erentiating between pollutants and providing high accuracy in measurement. These devices are prohibitively expensive and cannot easily be carried around. Due to the highly localized nature of air pollution, and in order to allow individuals and institutions to easily monitor their real-time exposure to PM2.5, we propose Halo, an air quality monitor costing less than $100. Halo is powered by a 500 mW solar panel and equipped with a 1500 mAh Lithium-Ion battery in order to handle 150 mW peak power consumption and operate continuously for over 24 hours without power input. The device is small enough to be clipped to a backpack or bag for easy portability, and it can be used in personal or public settings. Using an IR emitter and detector, Halo measures reflected IR light to determine the particulate concentration in the air with an error less than 10%. It uses Bluetooth Low Energy (BLE) to communicate these values to a user’s phone. From the phone, air data can be time-stamped, stored in a cloud database, and visualized in an app for easy monitoring of pollution trends and pollution exposure. Additionally, the cloud database allows for the aggregation of data from multiple devices to create crowdsourced pollution maps. These maps can be used to pinpoint areas with particularly bad air quality in order to try to make changes to these areas or to help users to know to avoid these areas in possible

First experiences with the implementation of the European standard EN 62304 on medical device software for the quality assurance of a radiotherapy unit

BACKGROUND: According to the latest amendment of the Medical Device Directive standalone software qualifies as a medical device when intended by the manufacturer to be used for medical purposes. In this context, the EN 62304 standard is applicable which defines the life-cycle requirements for the development and maintenance of medical device software. A pilot project was launched to acquire skills in implementing this standard in a hospital-based environment (in-house manufacture). METHODS: The EN 62304 standard outlines minimum requirements for each stage of the software life-cycle, defines the activities and tasks to be performed and scales documentation and testing according to its criticality. The required processes were established for the pre-existent decision-support software FlashDumpComparator (FDC) used during the quality assurance of treatment-relevant beam parameters. As the EN 62304 standard implicates compliance with the EN ISO 14971 standard on the application of risk management to medical devices, a risk analysis was carried out to identify potential hazards and reduce the associated risks to acceptable levels. RESULTS: The EN 62304 standard is difficult to implement without proper tools, thus open-source software was selected and integrated into a dedicated development platform. The control measures yielded by the risk analysis were independently implemented and verified, and a script-based test automation was retrofitted to reduce the associated test effort. After all documents facilitating the traceability of the specified requirements to the corresponding tests and of the control measures to the proof of execution were generated, the FDC was released as an accessory to the HIT facility. CONCLUSIONS: The implementation of the EN 62304 standard was time-consuming, and a learning curve had to be overcome during the first iterations of the associated processes, but many process descriptions and all software tools can be re-utilized in follow-up projects. It has been demonstrated that a standards-compliant development of small and medium-sized medical software can be carried out by a small team with limited resources in a clinical setting. This is of particular relevance as the upcoming revision of the Medical Device Directive is expected to harmonize and tighten the current legal requirements for all European in-house manufacturers

Different iron storage strategies among bloom-forming diatoms

Author Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here by permission of National Academy of Sciences for personal use, not for redistribution. The definitive version was published in Proceedings of the National Academy of Sciences of the United States of America 115(52), (2018): E12275-E12284. doi: 10.1073/pnas.1805243115.Diatoms are prominent eukaryotic phytoplankton despite being limited by the micronutrient iron in vast expanses of the ocean. As iron inputs are often sporadic, diatoms have evolved mechanisms such as the ability to store iron that enable them to bloom when iron is resupplied and then persist when low iron levels are reinstated. Two iron storage mechanisms have been previously described: the protein ferritin and vacuolar storage. To investigate the ecological role of these mechanisms among diatoms, iron addition and removal incubations were conducted using natural phytoplankton communities from varying iron environments. We show that among the predominant diatoms, Pseudo-nitzschia were favored by iron removal and displayed unique ferritin expression consistent with a long-term storage function. Meanwhile, Chaetoceros and Thalassiosira gene expression aligned with vacuolar storage mechanisms. Pseudo-nitzschia also showed exceptionally high iron storage under steady-state high and low iron conditions, as well as following iron resupply to iron-limited cells. We propose that bloom-forming diatoms use different iron storage mechanisms and that ferritin utilization may provide an advantage in areas of prolonged iron limitation with pulsed iron inputs. As iron distributions and availability change, this speculated ferritin-linked advantage may result in shifts in diatom community composition that can alter marine ecosystems and biogeochemical cycles.We thank the captain and crew of the R/V Melville and the CCGS J. P. Tully as well as the participants of the IRNBRU (MV1405) cruise for the California-based data, particularly K. Ellis [University of North Carolina (UNC)], T. Coale (University of California, San Diego), F. Kuzminov (Rutgers), H. McNair [University of California, Santa Barbara (UCSB)], and J. Jones (UCSB). W. Burns (UNC), S. Haines (UNC), and S. Bargu (Louisiana State University) assisted with sample processing and analysis. This work was funded by the National Science Foundation Grants OCE-1334935 (to A.M.), OCE-1334632 (to B.S.T.), OCE-1333929 (to K.T.), OCE-1334387 (to M.A.B.), OCE-1259776 (to K.W.B), and DGE-1650116 (Graduate Research Fellowship to R.H.L).2019-06-1

Transposition of the human Hsmar1 transposon: rate-limiting steps and the importance of the flanking TA dinucleotide in second strand cleavage

Hsmar1 is a member of the mariner family of DNA transposons. Although widespread in nature, their molecular mechanism remains obscure. Many other cut-and-paste elements use a hairpin intermediate to cleave the two strands of DNA at each transposon end. However, this intermediate is absent in mariner, suggesting that these elements use a fundamentally different mechanism for second-strand cleavage. We have taken advantage of the faithful and efficient in vitro reaction provided by Hsmar1 to characterize the products and intermediates of transposition. We report different factors that particularly affect the reaction, which are the reaction pH and the transposase concentration. Kinetic analysis revealed that first-strand nicking and integration are rapid. The rate of the reaction is limited in part by the divalent metal ion-dependent assembly of a complex between transposase and the transposon end(s) prior to the first catalytic step. Second-strand cleavage is the rate-limiting catalytic step of the reaction. We discuss our data in light of a model for the two metal ion catalytic mechanism and propose that mariner excision involves a significant conformational change between first- and second-strand cleavage at each transposon end. Furthermore, this conformational change requires specific contacts between transposase and the flanking TA dinucleotide